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Kajal N. Chauhan , Mandev B. Patel , Hima R. Valera , Savita D. Patil , Sanjay J. Surana

Abstract

The aqueous (100 mg/kg p.o.), methanolic (100 mg/kg p.o.) and petroleum ether (50 mg/kg p.o.) extracts of the flowers of Cassia auriculata linn, (Caesalpinaceae) were tested for their hepatoprotective activity against paracetamol induced hepatotoxicity in albino rats. Silymarin at a dose of 25 mg/kg i.p. was used as standard. The degree of protection was measured by using biochemical parameters like serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvate transaminase (SGPT), alkaline phosphatase (ALP), direct bilirubin and total bilirubin. The histopathological studies were also conducted. The aqueous and methanolic extracts of the flowers showed a significant hepatoprotective activity comparable with those of Silymarin.

Keywords

Hepatoprotective Activity, Cassia Auriculata, Paracetamol

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Vivekkumar K. Redasani ¹, Chetan F. Chhajed ¹, Pritam D. Patil ¹, Sanjay J. Surana ¹

Affiliations
1 Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur Dist. Dhule- 425 405 (MS), IN

Abstract

Two rapid, specific and economic methods have been developed using methanol as solvent for spectrophotometric determination of vilazodone HCl in bulk and in tablet formulation. Vilazodone HCl showed maximum absorption at 241nm (method A) and same spectrum was derivatised into first order derivative at Ae=2; amplitude of trough was recorded at 246nm (method B). In both methods vilazodone HCl follows linearity in concentration range 1-6μg/ml and exhibited good correlation coefficient (r²=0.999) and excellent mean recovery (98.32%) and (99.53%). The methods were validated statistically and by recovery studies for linearity, precision, repeatability, and reproducibility. The obtained results proved that both the methods can be employed for routine analysis of vilazodone HCl in bulks as well as in the commercial tablet formulation.

Keywords

Vilazodone Hcl, UV Spectroscopy, Derivative Spectroscopy, Validation.

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